Project

Part:BBa_K3113208

Designed by: Sarah Brajkovic, Joshua Hesse   Group: iGEM19_Munich   (2019-09-06)


pCAG_CD63_Ser161_6xHis_HiBit_MCP


This sequence encoded for CD63 fused to the HiBiT peptide and MCP. The 6xHis Tag inserted into the large extracellular loop of CD63 is used to facilitate purification of succesfully engineered exosomes. It is used to target fluc specifically into exosomes via MCP-MS2 connection. The HiBit peptide is used for detection via luminiscence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None